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INVESTIGATIONS ON THE USE OF THE ROLL TUBE METHOD FOR COUNTING ESCHERICHIA COLI I IN WATER
Author(s) -
PRETORIUS W. A.
Publication year - 1961
Publication title -
journal of applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0021-8847
DOI - 10.1111/j.1365-2672.1961.tb00254.x
Subject(s) - enumeration , escherichia coli , macconkey agar , agar , incubation , tube (container) , biology , microbiology and biotechnology , chromatography , chemistry , food science , mathematics , bacteria , materials science , biochemistry , genetics , combinatorics , gene , composite material
SUMMARY The roll tube method was used for the enumeration of Escherichia coli I in water and was found to be subject to less variation than the accepted method. Different media were compared and it was found that MacConkey's broth containing 3% of agar gave the best results. Pre‐incubation at 37% for 2 hr improved the selectivity and gave higher colony counts.