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A SELECTIVE MEDIUM FOR THE ISOLATION AND ENUMERATION OF COAGULASE POSITIVE STAPHYLOCOCCI FROM FOODS
Author(s) -
HOPTON J.
Publication year - 1961
Publication title -
journal of applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0021-8847
DOI - 10.1111/j.1365-2672.1961.tb00242.x
Subject(s) - coagulase , enumeration , isolation (microbiology) , microbiology and biotechnology , agar , cons , clearing , biology , yolk , staphylococcus , chemistry , food science , staphylococcus aureus , bacteria , mathematics , business , finance , computer science , genetics , combinatorics , programming language
SUMMARY A medium is described for the isolation and enumeration of coagulase positive staphylococci from food products. It was based on the observation that coagulase positive staphylococci produced a zone of clearing in egg yolk emulsion suspended in an agar medium. In conjunction with a modified coagulase test most coagulase positive staphylococci can be confirmed within 24 hr. The majority of coagulase negative staphylococci produced no clearing; when present it was frequently accompanied by a precipitation zone within the clearing area. Sodium azide was used as a selective agent.