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THE PRESERVATION OF MARINE BACTERIA
Author(s) -
FLOODGATE G. D.,
HAYES P. R.
Publication year - 1961
Publication title -
journal of applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0021-8847
DOI - 10.1111/j.1365-2672.1961.tb00237.x
Subject(s) - glycerol , bacteria , biology , food science , microbiology and biotechnology , biochemistry , genetics
SUMMARY Forty‐five marine bacteria and five nonmarine strains, representing 10 genera, were preserved by freeze drying, by storage in glycerol‐nutrient broth at —29° and under paraffin oil at 1°. Checks on viability and viable counts were made over a two year storage period. At the end of this time 100% of the strains had been preserved by freeze drying, 86% by storage in glycerol‐nutrient broth at low temperature and 72% under paraffin oil. The greatest loss of viability occurred among the vibrios and spirilla whichever of the three methods was used, but coryneforms, achromobacters and micrococci were successfully preserved by all these methods.