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STUDIES OF THE GROWTH OF PENICILLIUM CHRYSOGENUM IN CONTINUOUS FLOW CULTURE WITH REFERENCE TO PENICILLIN PRODUCTION
Author(s) -
PIRT S. J.,
CALLOW D. S.
Publication year - 1960
Publication title -
journal of applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0021-8847
DOI - 10.1111/j.1365-2672.1960.tb00181.x
Subject(s) - penicillium chrysogenum , mycelium , penicillin , growth rate , exponential growth , substrate (aquarium) , botany , volume (thermodynamics) , food science , chemistry , biology , microbiology and biotechnology , ecology , mathematics , thermodynamics , mathematical analysis , geometry , physics , antibiotics
SUMMARY: A filamentous mould was cultured by the continuous flow method in which medium is supplied at a constant rate and the culture volume is kept constant. Flow rates up to 0·1 culture volumes/hr were used. The mycelial dry weight concentration and the yield of mycelium/g of carbon source used were equal to or slightly greater than the maximum obtained in batch culture. With glucose concentrations up to 80 g/1. at a flow rate of 0·05 culture volumes/hr, about 45% of the substrate carbon was converted into mycelial carbon and the remainder oxidized to CO 2 . With unlimited amounts of all nutrients available growth of the mould followed the exponential law, as does bacterial growth, and therefore the mould had a constant doubling time. The oxygen demand of the mould as function of growth rate was determined. Conditions were found under which the rate of penicillin production/g of mycelium remained at its maximum value for 1000 hr.