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THE FORMATION OF GENTIOBIOSE AND OTHER SACCHARIDES DURING PRESSURE‐STEAM STERILIZATION OF A GLUCOSE‐SALTS SOLUTION OF pH 6.8
Author(s) -
KHAN A. W.,
WALKER T. K.
Publication year - 1958
Publication title -
journal of applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0021-8847
DOI - 10.1111/j.1365-2672.1958.tb00144.x
Subject(s) - chemistry , monosaccharide , chromatography , sterilization (economics) , biochemistry , monetary economics , economics , foreign exchange market , foreign exchange
SUMMARY: Two samples of glucose of high quality were examined chromatographically for impurities in the form of saccharides other than glucose. One contained gentiobiose and at least three other sugars amounting to 35 mg/100 g of glucose; the other contained gentiobiose and at least four other sugars, the total quantity being 25 mg/100 g of glucose. Glucose from which these small quantities of impurities had been removed was employed, together with inorganic salts, to make up a medium suitable for the cultivation of moulds and the pH value was adjusted to 6·8. This medium was sterilized at 10 lb pressure for 15 min and it was afterwards found that a mixture of saccharides amounting to 125 mg/100 g of glucose had been formed, the principal constituent being gentiobiose; the remainder consisted of a monosaccharide and two oligosaccharides not yet identified. These sugars were not detected in similar glucose salts media which had not been heated but which had been sterilized by Seitz fitration.