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THE EFFECT OF SUPPLEMENTATION OF THE GROWTH MEDIUM WITH YEAST EXTRACT, ON DEHYDROGENASE ACTIVITY OF ESCHERICHIA COLI
Author(s) -
HUGO W. B.,
LUND BARBARA M.
Publication year - 1958
Publication title -
journal of applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0021-8847
DOI - 10.1111/j.1365-2672.1958.tb00140.x
Subject(s) - escherichia coli , lactate dehydrogenase , dehydrogenase , yeast , biochemistry , yeast extract , enzyme , chemistry , formate dehydrogenase , mannitol , biology , fermentation , cofactor , gene
SUMMARY: Dehydrogenase activities have been determined of washed suspensions of Escherichia coli prepared from cultures grown in a synthetic medium, and in the same medium supplemented with yeast extract, under conditions of controlled aeration. This supplementation resulted in increased activity of enzymes attacking L (+)‐alanine, DL ‐serine, formate and succinate, no apparent change in activity of enzymes attacking L (+)‐gmlumate and D (—)‐ribose, and decreased action on D (+)‐glucose, mannitol and lactate. The results are discussed in the light of present knowledge of the metabolism of E. coli.