Premium
EXOCELLULAR PRODUCT FORMATION BY MICROORGANISMS IN CONTINUOUS CULTURE. I. PRODUCTION OF 2:3‐BUTANEDIOL BY AEROBACTER AEROGENES IN A SINGLE STAGE PROCESS
Author(s) -
PIRT S. J.,
CALLOW D. S.
Publication year - 1958
Publication title -
journal of applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0021-8847
DOI - 10.1111/j.1365-2672.1958.tb00134.x
Subject(s) - aerobacter aerogenes , dilution , yield (engineering) , sugar , 2,3 butanediol , butanediol , enterobacter aerogenes , chemistry , food science , chromatography , biochemistry , biology , fermentation , thermodynamics , escherichia coli , physics , gene
SUMMARY: A quantitative study of the growth and carbohydrate metabolism of Aerobacter aerogenes in continuous culture with particular reference to 2:3‐butanediol production is described. The effects of varying dilution rate, oxygen uptake rate, pH value, temperature and CO 2 pressure were studied. The optimum pH value was 5·0°6·0. The optimum temperature was 35–37° with a dilution rate of 0·2 hr −1 . The rate of sugar metabolism was decreased by raising the CO 2 pressure. In continuous culture the maximum diol output rate/1. of working volume exceeded the greatest rate reported for batch culture by a factor of three. The maximum diol concentration and yield as a percentage of the theoretical maximum were lower in continuous culture than in batch culture. Reasons for this are given. Some variants of the parent organism were found in continuous cultures but they caused no change in the yield of butanediol. There was no sign of loss of butanediol‐producing ability by any of the cultures, some of which were maintained for 2,000 hr. For the first few generations the rate of production of butanediol actually increased.