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THE USE OF STORED SUSPENSIONS OF ESCHERICHIA COLI I IN THE EVALUATION OF BACTERICIDAL ACTION
Author(s) -
COOK A. M.,
WILLS B. A.
Publication year - 1958
Publication title -
journal of applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0021-8847
DOI - 10.1111/j.1365-2672.1958.tb00133.x
Subject(s) - phenol , suspension (topology) , escherichia coli , potassium , bacteria , chromatography , aqueous suspension , chemistry , aqueous solution , microbiology and biotechnology , phosphate buffered saline , suspension culture , phosphate , biology , biochemistry , cell culture , organic chemistry , genetics , mathematics , homotopy , pure mathematics , gene
SUMMARY: Suspensions of Escherichia coli I, consisting of washed cells suspended in a phosphate buffer solution, maintained a higher viability and resistance to phenol than suspensions either of unwashed cells or of washed cells suspended in water. When stored for 5 weeks at room temperature, variations in their extinction times on exposure to aqueous phenol solutions were not significantly greater than variations with suspensions freshly prepared for each determination. Loss of resistance of a stored suspension to phenol, roxenol, lysol and potassium laurate was roughly parallel. Conditions of culture of the bacteria influenced the survival of suspensions, but the results indicated that pronounced differences may only be found in suspensions prepared from young cultures. The use of stored suspensions in the routine evaluation of bactericides is recommended.