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METHODS OF ASSESSING THE SPORICIDAL EFFICIENCY OF AN ULTRA‐HIGH‐TEMPERATURE MILK STERILIZING PLANT I. EXPERIMENTS WITH SUSPENSIONS OF SPORES IN WATER
Author(s) -
WILLIAMS D. J.,
FRANKLIN J. G.,
CHAPMAN HELEN R.,
CLEGG L. F. L.
Publication year - 1957
Publication title -
journal of applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0021-8847
DOI - 10.1111/j.1365-2672.1957.tb04514.x
Subject(s) - spore , sterilization (economics) , bacillus subtilis , nutrient agar , agar , nutrient , food science , incubation , chemistry , strain (injury) , endospore , agar plate , microbiology and biotechnology , biology , bacteria , biochemistry , genetics , organic chemistry , anatomy , monetary economics , economics , foreign exchange market , foreign exchange
SUMMARY: A method of assessing the sporicidal efficiency of a UHT milk sterilizing plant operating on water is described. Water heavily contaminated with spores of a strain of Bacillus subtilis was filtered, after treatment in the plant, through membrane filters and the surviving spores estimated by incubation of the membranes in nutrient agar. With this plant a temperature of c . 135° caused a 99·% kill of B. subtilis spores. Confirmation of the lethal effects of temperatures above 135° was obtained by passing treated water into 10 gal churns containing sterile concentrated nutrient broth and incubating the churns.