Premium
THE DIFFERENTIATION OF BACTERIAL SPECIES BY PAPER CHROMATOGRAPHY. IV. AN EXAMINATION OF THE MICROCOCCI, WITH SPECIAL REFERENCE TO THE INFLUENCE ON THE CHROMATOGRAMS OF MEDIUM AND AGE OF CULTURE
Author(s) -
GREGORY MARGARET,
MABBITT L. A.
Publication year - 1957
Publication title -
journal of applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0021-8847
DOI - 10.1111/j.1365-2672.1957.tb00096.x
Subject(s) - composition (language) , chromatography , chemistry , paper chromatography , bacterial growth , bacteria , food science , biology , genetics , philosophy , linguistics
SUMMARY: The amino acid and peptide content of the micrococci as revealed by paper chromatography depended on the physiological age of the culture and on the composition of the growth medium. The use of different peptones in a Yeastrel glucose peptone medium resulted in minor changes in the chromatograms. A greater effect was noted when glucose was omitted. When the composition of the growth medium was standardized the best comparison of cultures was afforded by the preparation of chromatograms from samples taken in the late logarithmic and early stationary phases of growth.