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A TWO LITRE SCALE CONTINUOUS CULTURE APPARATUS FOR MICRO‐ORGANISMS
Author(s) -
ELSWORTH R.,
MEAKIN L. R. P.,
PIRT S. J.,
CAPELL G. H.
Publication year - 1956
Publication title -
journal of applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0021-8847
DOI - 10.1111/j.1365-2672.1956.tb00077.x
Subject(s) - aeration , bottle , defoamer , mixing (physics) , volumetric flow rate , process engineering , continuous flow , environmental science , materials science , mechanical engineering , pulp and paper industry , mechanics , engineering , waste management , biochemical engineering , physics , dispersion (optics) , optics , quantum mechanics , dispersant
SUMMARY: The design and construction of a continuous culture apparatus with a 2 1. culture vessel are described. Aeration is achieved by means of a mechanical stirrer and injected air, automatic temperature and pH control are features of the apparatus, and foaming is controlled by adding antifoam through a manually operated valve. A high degree of agitation ensures that good mixing takes place. Accurate and easily variable control of the medium flow rate is obtained by means of the Mariotte bottle principle. The apparatus can be operated continuously with freedom from contamination for periods in excess of 1000 hr.