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THE DETERMINATION OF COLI‐AEROGENES ORGANISMS IN RAW MILK BY SURFACE SMEARS ON DRIED EOSIN METHYLENE BLUE AGAR PLATES
Author(s) -
GRIFFITHS D. G.,
POWYSDAVIES ELUNED,
THOMAS S. B.
Publication year - 1956
Publication title -
journal of applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0021-8847
DOI - 10.1111/j.1365-2672.1956.tb00037.x
Subject(s) - methylene blue , agar , bacteria , eosin y , serial dilution , raw milk , staining , agar plate , chemistry , chromatography , eosin , escherichia coli , food science , microbiology and biotechnology , biology , biochemistry , medicine , alternative medicine , pathology , photocatalysis , gene , genetics , catalysis
SUMMARY: The determination of the coli‐aerogenes content of raw milk by smearing 0·1 or 0·2 ml of suitable dilutions on dried Levine's eosin methylene blue agar plates and incubating at 37° or 30°, was found too unselective as a routine technique. Only about two‐thirds of the colonies showing the characteristic appearance of coli‐aerogenes bacteria were confirmed as such by the formation of acid and gas in MacConkey's broth. Appreciable proportions of the numerous rose coloured colonies and of the very small dark colonies with metallic sheen, which are not considered to be coliaerogenes bacteria, formed acid and gas in MacConkey's broth.