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STUDIES OF THE DETERMINATION OF THE BACTERIUM COLI TYPE I CONTENT OF FARM WATER SUPPLIES
Author(s) -
THOMAS S. B.,
CLEGG L. F. L.,
CUTHBERT W. A.,
OXLEY C. D.,
SCARLETT C. A.,
WESTWATER C. H.,
JONES G. ELIS,
McCLEMONT J.,
MORRIS C. S.,
NICHOLS AGNES A.
Publication year - 1955
Publication title -
journal of applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0021-8847
DOI - 10.1111/j.1365-2672.1955.tb02055.x
Subject(s) - bacteria , escherichia coli , indole test , microbiology and biotechnology , biology , chemistry , food science , stereochemistry , biochemistry , genetics , gene
SUMMARY: A – comparison of the suitability of brilliant green bile broth and MacConkey's broth at 44° for the detection of Bacterium coli type I in farm water supplies, showed that 83.1% of the samples had no difference in the number of positive tubes at 44°, and only 5 samples (1.7%) had a significantly higher number of positive tubes in MacConkey's broth. Of 707 strains of coli‐aerogenes bacteria isolated from 44° positive tubes of both media, 94.5% were Bact. coli type I. Strains of Bact. coli type II and Bact. aerogenes type I which were 44° positive constituted 3.7% and 0.4% respectively, all of which were indole negative at 44°. In addition there were 10 strains (1.4%) of 44° positive Intermediate type II, 9 of which were indole positive at 44°. An appreciable number (6.6%) of Bact. coli type I strains failed to give a positive indole reaction in 24 hr at 44°.