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THE ENUMERATION OF LACTOBACILLI ON GRASS AND IN SILAGE
Author(s) -
KEDDIE R. M.
Publication year - 1951
Publication title -
proceedings of the society for applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0370-1778
DOI - 10.1111/j.1365-2672.1951.tb04627.x
Subject(s) - silage , food science , agar , acetic acid , sodium acetate , chemistry , enumeration , sterilization (economics) , acetone , chromatography , bacteria , biology , biochemistry , mathematics , genetics , combinatorics , monetary economics , economics , foreign exchange market , foreign exchange
SUMMARY: For the enumeration of lactobacilli on grass and in silage the following medium has shown promise: peptone, meat extract and glucose, 10 g. each; tomato extract, 200 ml.; yeast autolysate, 50 ml.; Tween 80, 0.5 ml.; agar, 15 g., in a final volume of 1 1. and containing acetic acid/sodium acetate buffer in 0.2M concentration; pH 5–4. The medium was adjusted to pH 5–4 before sterilization and the requisite amount of concentrated pH 5.4 acetate buffer added just before plating. Double laver plates were used. The only other silage organisms which in this medium formed colonies comparable in size with those of lactobacilli were heterofermentative streptococci and a micrococcus.