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Detection of clenbuterol in beef meat, liver and kidney by mid‐infrared spectroscopy (FT‐Mid IR) and multivariate analysis
Author(s) -
MezaMárquez Ofelia G.,
GallardoVelázquez Tzayhrí,
OsorioRevilla Guillermo,
DorantesÁlvarez Lidia
Publication year - 2012
Publication title -
international journal of food science and technology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.831
H-Index - 96
eISSN - 1365-2621
pISSN - 0950-5423
DOI - 10.1111/j.1365-2621.2012.03108.x
Subject(s) - clenbuterol , chemometrics , chemistry , detection limit , chicken liver , food science , chromatography , biochemistry , enzyme
Summary Mid‐infrared spectroscopy ( FT ‐Mid IR ) coupled with multivariate analysis was used to predict clenbuterol in beef meat, liver and kidney. A SIMCA model was also developed to discriminate between pure (beef meat, liver and kidney) and spiked with clenbuterol samples (beef meat‐clenbuterol, liver‐clenbuterol and kidney‐clenbuterol). The best models to predict clenbuterol concentrations were obtained using the partial least squares algorithm ( PLS ) with a R 2 > 0.9 and SEC and standard error of prediction <0.296 and 0.324, respectively. The SIMCA model used to discriminate pure and spiked with clenbuterol samples showed 100% correct classification rate. Methods detection limit was 2 μg kg −1 . FT‐Mid IR coupled with chemometrics could be a simple and rapid screening tool for monitoring clenbuterol in beef meat, liver and kidney implicated in food poisoning. This method could be use for screening purposes.