Premium
Antioxidant and trypsin inhibition activity of dioscorin influenced by yam cultivars and eluent pH
Author(s) -
Kao WenTzu,
Chang ChenTien,
Lin KuoWei
Publication year - 2012
Publication title -
international journal of food science and technology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.831
H-Index - 96
eISSN - 1365-2621
pISSN - 0950-5423
DOI - 10.1111/j.1365-2621.2012.02984.x
Subject(s) - cultivar , chemistry , antioxidant , column chromatography , trypsin , gel permeation chromatography , isoelectric point , isoelectric focusing , trypsin inhibitor , fractionation , food science , chromatography , biochemistry , botany , biology , enzyme , polymer , organic chemistry
Summary The total antioxidant activity (TAA) and trypsin inhibition activity (TIA) of storage protein dioscorin from six yam cultivars (Dashan, Hualien No. 3, Keelung, Mingchien, Tainong No. 2, and Japanese yam) were demonstrated. Japanese yam had the highest TAA and the lowest calculated EC 50 at any purification stage. Following gel permeation, dioscorins purified from Hualien No. 3 and Japanese yam showed the highest TIA and lowest calculated IC 50 . Dioscorin with higher TIA apparently possesses better TAA. The pIs of dioscorin from all yam species were in the range of 5.94–4.33 indicating acidic protein in nature. Results from isoelectric focusing chromatography showed regardless of species higher TIA for dioscorin eluted at lower pH.