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Antioxidant and DNA protection activities of a glycoprotein isolated from a seaweed, Saccharina japonica
Author(s) -
Kim EunYoung,
Kim YuRi,
Nam TaekJeong,
Kong InSoo
Publication year - 2012
Publication title -
international journal of food science and technology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.831
H-Index - 96
eISSN - 1365-2621
pISSN - 0950-5423
DOI - 10.1111/j.1365-2621.2012.02936.x
Subject(s) - antioxidant , chemistry , pronase , biochemistry , dpph , ethylenediaminetetraacetic acid , abts , nitric oxide , food science , enzyme , chelation , organic chemistry , trypsin
Summary A glycoprotein with a molecular weight of ∼10 kDa was purified from Saccharina japonica , and its antioxidant activity was evaluated using five different antioxidant assays. The highest antioxidant activities of the purified glycoprotein determined by DPPH, ABTS, SODA, SORS and FRAP assays were 85%, 95%, 94%, 72% and 97%, respectively. About 37% nitric oxide scavenging and 85% xanthine oxidase inhibition activities were also observed at a concentration of 5 mg mL −1 . The glycoprotein exhibited optimal activity levels of free radical scavenging at pH 7–9 and 40 °C. Antioxidant activity decreased when treated with ethylenediaminetetraacetic acid and metal ions. Moreover, its scavenging activity decreased when the glycoprotein was treated with deactivation agents such as pronase E or sodium periodate (NaIO 4 ), and it showed protective activity against DNA cleavage induced under oxidative conditions.