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Optimal conditions for the production of monoacylglycerol from crude palm oil by an enzymatic glycerolysis reaction and recovery of carotenoids from the reaction product
Author(s) -
Majid Norholis,
Cheirsilp Benjamas
Publication year - 2012
Publication title -
international journal of food science and technology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.831
H-Index - 96
eISSN - 1365-2621
pISSN - 0950-5423
DOI - 10.1111/j.1365-2621.2011.02909.x
Subject(s) - monoacylglycerol lipase , chemistry , glycerol , chromatography , lipase , yield (engineering) , hexane , carotenoid , monoglyceride , organic chemistry , food science , enzyme , biochemistry , endocannabinoid system , receptor , materials science , fatty acid , metallurgy
Summary Monoacylglycerol (MAG) was produced from crude palm oil (CPO) by the enzymatic glycerolysis reaction in organic solvents. The optimal conditions for MAG production from CPO were: the use of a mixture of tert‐butanol and hexane (1:1) as the organic solvents; an immobilised lipase of 40%; a molar ratio of glycerol to CPO of 8:1 with 4% of water content in the glycerol; and an initial CPO concentration of 10%. A maximum yield of 74.3% MAG was obtained with an initial production rate of 42.3 mg MAG mL −1 h −1 . By converting CPO to the more polar MAG, the efficiency of recovery of carotenoids by adsorption column chromatography was improved up to 75.7% from 55.1% when using unconverted CPO. This study provides information that will be useful for developing an efficient and cheaper industrialised process for the production of MAG from CPO and recovery of carotenoids from the reaction product.