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Interesterification of milkfat with soybean oil catalysed by Rhizopus oryzae lipase immobilised on SiO 2 ‐PVA on packed bed reactor
Author(s) -
Paula Ariela V.,
Nunes Gisele F. M.,
Santos Júlio C.,
de Castro Heizir F.
Publication year - 2011
Publication title -
international journal of food science and technology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.831
H-Index - 96
eISSN - 1365-2621
pISSN - 0950-5423
DOI - 10.1111/j.1365-2621.2011.02726.x
Subject(s) - butterfat , interesterified fat , rhizopus oryzae , lipase , chemistry , soybean oil , packed bed , chromatography , food science , rhizopus , organic chemistry , linseed oil , enzyme , milk fat , fermentation
Summary The potential of the lipase from Rhizopus oryzae immobilised on SiO 2 ‐PVA to catalyse the interesterification of the milkfat with soybean oil in a packed bed reactor running on continuous mode was evaluated. The reactor operated continuously for 35 days at 45 °C, and during 12 days, no significant decrease in the initial lipase activity was verified. Interesterification yields were in the range from 35 to 38%wt, which gave an interesterified product having 59% lower consistency in relation to non‐interesterified blend. Results showed the potential of the lipase from Rhizopus oryzae to mediate the interesterification of milkfat with soybean oil in packed bed reactor, attaining a more spreadable product under a cool temperature. The biocatalyst operational stability was assessed and an inactivation profile was found to follow the Arrhenius model, revealing values of 34 days and 0.034 day −1 , for half‐life and a deactivation coefficient, respectively.