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Production of structured lipids containing conjugated linolenic acid: optimisation by response surface methodology
Author(s) -
Elibal Begum,
Suzen H. Funda,
Aksoy H. Ayse,
Ustun Guldem,
Tuter Melek
Publication year - 2011
Publication title -
international journal of food science and technology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.831
H-Index - 96
eISSN - 1365-2621
pISSN - 0950-5423
DOI - 10.1111/j.1365-2621.2011.02635.x
Subject(s) - bitter gourd , chemistry , response surface methodology , linolenic acid , yield (engineering) , food science , lipase , molar ratio , substrate (aquarium) , fatty acid , palm stearin , hexane , chromatography , enzyme , organic chemistry , momordica , catalysis , linoleic acid , palm oil , materials science , biology , medicine , ecology , metallurgy , traditional medicine
Summary Structured lipids containing conjugated linolenic acid (CLNA) were produced separately by enzymatic acidolysis reaction of corn and canola oils (CAO) with bitter gourd ( Momordica charantia L) seed oil fatty acids [bitter gourd seed oil fatty acids (BGFA)]. Reactions were conducted using a commercial immobilised sn‐1,3‐specific lipase from Thermomyces lanuginosa (Lipozyme TL IM) in hexane. The effects of reaction time, substrate molar ratio, temperature and enzyme amount on incorporation yield of CLNA were investigated and optimised by response surface methodology with three‐level, two‐factor face‐centred cube design. When reactions were conducted using 10% enzyme for 3 h, the optimum reaction conditions were found for corn oil (CO) as 53.5 °C and 5.9:1 BGFA/CO molar ratio. At these conditions, the incorporation of CLNA into CO was determined as 41.4%. However, CLNA incorporation into CAO was resulted as 37% at optimum conditions which were 54.2 °C and 6.8:1 BGFA/CAO molar ratio.