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Study on rapid quantitative detection of total bacterial counts by the ATP‐bioluminescence and application in probiotic products
Author(s) -
Wu Huiqing,
Wu Qingping,
Zhang Jumei,
Li Chengsi,
Huang Ziguang
Publication year - 2011
Publication title -
international journal of food science and technology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.831
H-Index - 96
eISSN - 1365-2621
pISSN - 0950-5423
DOI - 10.1111/j.1365-2621.2010.02531.x
Subject(s) - bioluminescence , probiotic , viable count , plate count , adenosine triphosphate , chemistry , luciferase , biochemistry , chromatography , microbiology and biotechnology , bacteria , biology , genetics , fermentation , transfection , gene
Summary The objective of this study was to develop a rapid quantitative detection method by triphosphate (ATP)‐bioluminescence and determine the feasibility to assay total bacterial counts (TBC) in probiotic products. A useful pretreatment technique to eliminate the interference to the ATP‐bioluminescence method was developed, resulting in a 10–100‐fold improvement in the rapid quantitative detection method by ATP‐bioluminescence. The TBC obtained by the ATP‐bioluminescence rapid detection method was tested against the direct microscopic count method or the plate count method and validated on three ATP Assay Systems. The results generated by the ATP methods and the plate count method were comparable to each other when the interference due to non‐microbial cell ATP and matter such as pigment were removed. This study reports the optimisation of an ATP‐bioluminescence assay using a pretreatment technique to eliminate interference in order to quickly determine the viable counts of bacteria in solid and liquid probiotic products.