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Optimisation of porous starch preparation by ultrasonic pretreatment followed by enzymatic hydrolysis
Author(s) -
Qian Junqing,
Chen Xingyu,
Ying Xuexiao,
Lv Binfeng
Publication year - 2011
Publication title -
international journal of food science and technology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.831
H-Index - 96
eISSN - 1365-2621
pISSN - 0950-5423
DOI - 10.1111/j.1365-2621.2010.02469.x
Subject(s) - hydrolysis , starch , amylase , enzymatic hydrolysis , response surface methodology , chemistry , chromatography , scanning electron microscope , raw material , alpha amylase , enzyme , corn starch , porosity , materials science , biochemistry , organic chemistry , composite material
Summary Porous starch granules were formed by the partial hydrolysis of starch using amylase. Fungal amylase and corn starch were chosen as the original amylase and substance from different sources, respectively. Ultrasonic technique as an assistant of enzymatic hydrolysis was used to pretreat raw starch. Scanning electron microscopy was used to examine the structure of native and treated starch, revealing the size of the pores in each occasion. The extent of enzymatic hydrolysis ( y %) was markedly enhanced by statistical optimisation of enzymatic conditions. A significant influence of amount, temperature and pH of enzyme has been noted with Plackett–Burman design. It was then revealed with response surface methodology (RSM) that 503.26 U (g substance) −1 amount of enzyme, temperature of 55 °C and pH of 5.1 were optimum. This optimisation strategy led to the enhancement of y % from 53.4% to 61.38%.