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Isolation of DNA from genetically modified oils by fast protein liquid chromatography
Author(s) -
Huang Li,
Chen Yan,
Wang Ya,
Pan Jian,
Tong Yuan
Publication year - 2010
Publication title -
international journal of food science and technology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.831
H-Index - 96
eISSN - 1365-2621
pISSN - 0950-5423
DOI - 10.1111/j.1365-2621.2010.02295.x
Subject(s) - fast protein liquid chromatography , chromatography , chemistry , agarose gel electrophoresis , dna , agarose , ion chromatography , absorbance , gel electrophoresis , high performance liquid chromatography , electrophoresis , polymerase chain reaction , gel electrophoresis of nucleic acids , biochemistry , gene
Summary In this study, a novel method of fast protein liquid chromatography (FPLC) anion exchange chromatography was developed for isolation of DNA from processed genetically modified (GM) oils. Four kinds of different GM edible oil had been chosen as model sample. Salmon DNA was used as the control sample to determine the pH values and NaCl in mobile phase buffer. Applying pH 8 and NaCl gradient 0.5–2 m were chosen for the DNA isolation. The quality and purity of isolated DNA were tested with agarose gel electrophoresis, scanned with UV absorbance spectra and amplified by polymerase chain reaction (PCR). The result indicated that the quantity of DNA isolated by FPLC was suitable for further PCR analyses. Furthermore, it is more effective and less time‐consuming in comparison with cetyltrimethylammonium bromide method and High Pure GMO Sample Preparation Kit method.