Premium
Enantioselective determination of cypermethrin in pig muscle tissue by immunoaffinity extraction and high performance liquid chromatography
Author(s) -
Kuang Hua,
Miao Hong,
Wu Yongning,
Shen Jianzhong,
Xu Chuanlai
Publication year - 2010
Publication title -
international journal of food science and technology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.831
H-Index - 96
eISSN - 1365-2621
pISSN - 0950-5423
DOI - 10.1111/j.1365-2621.2010.02192.x
Subject(s) - chromatography , cypermethrin , chemistry , enantiomer , elution , extraction (chemistry) , hexane , isopropyl alcohol , high performance liquid chromatography , pesticide , biology , stereochemistry , organic chemistry , agronomy
Summary A high performance liquid chromatography method was developed to detect enantiomers of cypermethrin in pork muscle. Eight isomers of racemic cypermethrin were separated using a normal mobile phase of hexane–isopropyl alcohol (99.3–0.7, v/v) on a chiral CD‐ph column in 25 min. The elution order of isomers was ascertained using enantiomer/diastereomer enriched cypermethrin standards. The extracts from pork samples were cleaned up by immunoaffinity column before detection. The recoveries from samples fortified levels of 0.1 and 1 mg kg −1 ranged from 67.3% to 113.5% with a relative standard deviation 6.7–16.8%; sufficient to effectively measure cypermethrin enantiomer concentrations in pig muscle tissue samples in the future.