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Evaluation of the conditions of carotenoids production in a synthetic medium by Sporidiobolus salmonicolor (CBS 2636) in a bioreactor
Author(s) -
Valduga Eunice,
Tatsch Pihetra Oliveira,
Tiggemann Lídia,
Zeni Jamile,
Colet Rosicler,
Cansian João Marcos,
Treichel Helen,
Luccio Marco
Publication year - 2009
Publication title -
international journal of food science and technology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.831
H-Index - 96
eISSN - 1365-2621
pISSN - 0950-5423
DOI - 10.1111/j.1365-2621.2009.02032.x
Subject(s) - carotenoid , bioreactor , food science , chemistry , fermentation , aeration , substrate (aquarium) , botany , biology , organic chemistry , ecology
Summary This work studied the cultivation conditions for the production of carotenoids by Sporidiobolus salmonicolor (CBS 2636) in a bioreactor. A Plackett–Burman design was used for the screening of the most important factors, followed by a complete second order design, to maximise the concentration of total carotenoids. The maximum concentration of 3425.9 μg L −1 of total carotenoids was obtained in a medium containing 80 g L −1 glucose, 15 g L −1 peptone and 5 g L −1 malt extract, with an aeration rate 1.5 vvm, 180 r.p.m., 25 °C and an initial pH of 4.0. Fermentation kinetics showed that the maximum concentration of total carotenoids was reached after 90 h of fermentation. Carotenoid bio‐production was partially associated with cell growth. The specific carotenoid production ( Y P/X ) was 238 μg carotenoids/g cells, whereas Y P/S (substrate to product yield) was 41.3 μg g −1 . The specific growth rate ( μ x ) was 0.045 h −1 . The highest cell and total carotenoid productivity were 0.19 g L −1 h −1 and 56.9 μg L −1 h −1 , respectively.