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Angiotensin I‐converting enzyme inhibitory activity of Acetes chinensis peptic hydrolysate and its antihypertensive effect in spontaneously hypertensive rats
Author(s) -
Zhang Chaohua,
Cao Wenhong,
Hong Pengzhi,
Ji Hongwu,
Qin Xiaoming,
He Jinfeng
Publication year - 2009
Publication title -
international journal of food science and technology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.831
H-Index - 96
eISSN - 1365-2621
pISSN - 0950-5423
DOI - 10.1111/j.1365-2621.2009.02028.x
Subject(s) - hydrolysate , ic50 , pharmacology , chemistry , inhibitory postsynaptic potential , renin–angiotensin system , ultrafiltration (renal) , antihypertensive drug , enzyme , angiotensin converting enzyme , peptide , spontaneously hypertensive rat , endocrinology , medicine , biochemistry , blood pressure , in vitro , hydrolysis
Summary Peptic hydrolysate with angiotensin I‐converting enzyme (ACE) inhibitory activity was prepared from Acetes chinensis . The 3 kDa ultrafiltration filtrates (UF‐IV) of the desalted hydrolysate exerted a potent ACE inhibitory activity with IC50 being 0.67 mg mL −1 . The fractions with molecular weight located between 611 and 240 Da achieved a total percentage of 63.6% in the UF‐IV, with a mean peptide length of about 5.6–2.1. Gastrointestinal digestions were simulated on the hydrolysate and UF‐IV and addressed their effect on the ACE inhibitory activity. The ACE inhibitory activity of the UF‐IV could survive or be maintained in active form by simulated gastrointestinal digestion. Furthermore, the UF‐IV exerted potent antihypertensive effect after oral administrations were given to spontaneously hypertensive rats (SHR) at a dose of 900 mg kg −1 body weight. In conclusion, the UF‐IV could serve as useful antihypertensive peptides in the prevention and treatment of hypertension and other associated disorders.