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A LightCycler TaqMan PCR assay for quantitative detection of chamois ( Rupicapra rupicapra ) and pyrenean ibex ( Capra pyrenaica ) in experimental meat mixtures
Author(s) -
Fajardo Violeta,
González Isabel,
Martín Irene,
Rojas María,
Hernández Pablo E.,
García Teresa,
Martín Rosario
Publication year - 2009
Publication title -
international journal of food science and technology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.831
H-Index - 96
eISSN - 1365-2621
pISSN - 0950-5423
DOI - 10.1111/j.1365-2621.2009.02020.x
Subject(s) - taqman , biology , dna , veterinary medicine , real time polymerase chain reaction , 18s ribosomal rna , microbiology and biotechnology , gene , ribosomal rna , genetics , medicine
Summary Accurate quantitative assays are required for enforcing food labelling procedures and preventing food ingredient contamination, misdescription and fraud. Two species‐specific TaqMan systems and a eukaryotic endogenous TaqMan system were combined in a real time PCR approach to achieve quantification of chamois and pyrenean ibex meats in meat mixtures. The caprinae‐specific systems were based on the amplification of a 133 bp fragment of the 12S rRNA gene from chamois DNA, whereas an 88 bp fragment from the D‐loop region was amplified from pyrenean ibex DNA. Universal primers amplified a 141 bp fragment on the nuclear 18S rRNA gene from eukaryotic DNA. Analysis of experimental raw and heat treated binary mixtures of chamois and pyrenean ibex meat in a swine meat matrix demonstrated the suitability of the assay for the detection and quantification of the target DNAs in the range of 0.1–25%, depending on the species and treatment of the meat samples.