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Screening of cereal varieties for antioxidant and radical scavenging properties applying various spectroscopic and thermoanalytical methods
Author(s) -
Brindzová Lucia,
Zalibera Michal,
Šimon Peter,
Čertík Milan,
Takácsová Mária,
Mikulajová Anna,
Mikušová Lucia,
Rapta Peter
Publication year - 2009
Publication title -
international journal of food science and technology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.831
H-Index - 96
eISSN - 1365-2621
pISSN - 0950-5423
DOI - 10.1111/j.1365-2621.2008.01898.x
Subject(s) - dpph , abts , trolox , chemistry , antioxidant , food science , hordeum vulgare , antioxidant capacity , electron paramagnetic resonance , botany , organic chemistry , biology , poaceae , nuclear magnetic resonance , physics
Summary Twenty varieties of four representative cereals, and sixteen varieties of six pseudocereals were investigated by different assays and analyses, covering the quantitative analysis of antioxidants, radical scavenging capacity (RSC) as well as total antioxidant capacity of cereals. The inter‐variety variability in antioxidant capacity was determined by four independent methods for evaluation of antioxidant activity including 2,2′‐azinobis‐(3‐ethylbenzthiazoline)‐6‐sulphonic acid (ABTS) and 2,2‐diphenyl‐1‐picrylhydrasyl (DPPH) tests, electron paramagnetic resonance (EPR)/spin‐trapping assay and differential thermal analysis (DTA). The antioxidant capacity of studied samples determined in ABTS test ranged from 0.70 to 7.33 mg Trolox g −1 , in DPPH test from 1.31 to 54.79 mg Trolox g −1 and RSC values from EPR/spin‐trapping assay varied from 0.27 to 13.52 mg Trolox g −1 dry matter. The best antioxidant capacity was found for buckwheat ( Fagopyrum esculentum Moench), barley ( Hordeum vulgare L.) and Japanese millet ( Echinochloa frumentacea L.), where also the highest contents of total phenolics and exceptional thermal stability were observed.