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Screening for natural defence mechanisms of Lactococcus lactis strains isolated from traditional starter cultures
Author(s) -
Miklič Andreja,
Rogelj Irena
Publication year - 2007
Publication title -
international journal of food science and technology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.831
H-Index - 96
eISSN - 1365-2621
pISSN - 0950-5423
DOI - 10.1111/j.1365-2621.2007.01175.x
Subject(s) - lactococcus lactis , starter , microbiology and biotechnology , biology , bacteriophage , gene , bacteria , lactococcus , nuclease , polysaccharide , food science , genetics , biochemistry , lactic acid , escherichia coli
Summary Three different bacterial defence mechanisms were identified in the seventeen Lactococcus lactis isolates from starter cultures in three Slovenian dairy plants. Isolates MB18, KR7, PT4, PT13 and PT19 inhibited phage adsorption by means of exopolysaccharides production. The most extensive polysaccharides production was detected in PT19 isolate, which was susceptible only to phage ΦPT19. Eight isolates exhibited nuclease activity, and seven of them were susceptible up to four phages out of thirteen from our collection. Eight isolates possessed the abi B gene, fourteen isolates abi H, two isolates abi J and one isolate abi Q. Isolates PT27 and PT28 possessed AbiB, AbiH and AbiJ mechanisms as well as inhibition of phage adsorption. Isolate MB18, which was susceptible to one phage only, possessed the abi Q gene, nuclease activity and ability to prevent adsorption of most phages. Isolates PT67 and PT70, possessing only AbiH mechanism, were susceptible to only two phages.