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Induction of Quinone Reductase, The Phase 2 Anticarcinogenic Marker Enzyme, in Hepa1c1c7 Cells by Radish Sprouts, Raphanus sativus L.
Author(s) -
Lee SyngOok,
Lee InSeon
Publication year - 2006
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/j.1365-2621.2006.tb08917.x
Subject(s) - reductase , carcinogen , enzyme inducer , raphanus , chemistry , dicoumarol , enzyme , biochemistry , inducer , quinone , nad+ kinase , nicotinamide adenine dinucleotide , microbiology and biotechnology , biology , gene , botany
The activity of a methanol extract of radish sprouts for the induction of nicotinamide adenine dinucleotide (phosphate) NAD(P)H/quinone reductase (QR), which plays critical roles in protection against chemical carcinogens and other toxic xenobiotics, was examined in murine Hepa1c1c7 cells. The methanol extract induced QR activity in a dose‐dependent manner in the concentration range of 0.2 to 1.6 mg/mL with a maximum of a 3.5‐fold increase in induction. The induction of QR by the extract was regulated at the transcriptional level. Using a Western blotting analysis and Ah‐receptor‐defective mutant of Hepa1c1c7 cells (BP r c1 cells), the extracts at a concentration of 0.8 mg/mL or lower was found to be a monofunctional inducer and caused no elevation in cytochrome P‐450 level that may activate carcinogens. The dichloromethane (CH 2 Cl 2 ) fraction of the extract showed the highest induction potency while the other fractions were less potent. These results indicate that radish sprouts can be regarded as a safe and promising new dietary source for decreasing the risk of developing cancer.