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Inhibition of Listeria monocytogenes by Nisin Combined with Grape Seed Extract or Green Tea Extract in Soy Protein Film Coated on Turkey Frankfurters
Author(s) -
Theivendran Sivarooban,
Hettiarachchy Navam S.,
Johnson Michael G.
Publication year - 2006
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/j.1365-2621.2006.tb08905.x
Subject(s) - nisin , food science , listeria monocytogenes , green tea extract , grape seed extract , chemistry , population , inoculation , antimicrobial , bacteria , green tea , biology , horticulture , medicine , genetics , alternative medicine , demography , organic chemistry , pathology , sociology
The objective of this study was to evaluate the inhibitory effect of grape seed extract (GSE), green tea extract (GTE), nisin and their combinations (nisin with either GSE or GTE) against Listeria monocytogenes . The inhibitory effect of these natural compounds was evaluated in phosphate buffer solution (PBS) medium containing approximately 10 9 colony‐forming units (CFU/mL) of L. monocytogenes. The effectiveness of these compounds in a meat model system was evaluated by surface inoculation (approximately 10 6 CFU/g) of L. monocytogenes onto turkey frankfurters. The inoculated frankfurters were dipped into soy protein film‐forming solutions with and without the addition of antimicrobial agents (GSE 1% or GTE 1% or nisin 10000 IU or combinations). Samples were stored at either 4 °C or 10 °C. The inhibitory effects of edible coatings were evaluated on a weekly basis for 28 d. The greatest inhibitory effect was observed in the PBS medium containing GSE (1%) and nisin (10000 IU/mL), which caused a 9‐log cycle reduction of L. monocytogenes population after 3 h incubation at 37 °C. In the meat system, the L. monocytogenes population (7.1 CFU/g) was decreased by more than 2 log cycle after 28 d at 4 °C and 10 °C, in the samples containing nisin (10000 IU) combined with either GSE (1%) or GTE (1%). This research has demonstrated that the use of an edible film coating containing both nisin and natural extracts is a promising means of controlling the growth and recontamination of L. monocytogenes on ready‐to‐eat meat products.