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Determination of the veterinary drug maduramicin in food by fluorescence polarisation immunoassay
Author(s) -
Wang Zhanhui,
Zhang Suxia,
Murtaziilya R.,
Eremin Sergei A.,
Shen Jianzhong
Publication year - 2008
Publication title -
international journal of food science and technology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.831
H-Index - 96
eISSN - 1365-2621
pISSN - 0950-5423
DOI - 10.1111/j.1365-2621.2006.01400.x
Subject(s) - polyclonal antibodies , antiserum , detection limit , chromatography , immunoassay , chemistry , bovine serum albumin , fluorescein , fluorescence polarization immunoassay , fluorescence , antibody , biology , immunology , physics , quantum mechanics
Summary A fluorescence polarisation immunoassay (FPIA) using a specific polyclonal antiserum for the detection of maduramicin (MD) was developed and optimised. The polyclonal antiserum was produced against MD linked to bovine serum albumin. Fluorescein‐labelled MD (tracer) was synthesised by N ‐hydroxysuccinimide active ester method and purified using thin layer chromatography. The developed FPIA for MD had a dynamic range from 0.01 to 5.6 μ g mL −1 with an IC 50 value of 0.16 μ g mL −1 and a limit of detection of 0.002 μ g mL −1 . Recoveries from chicken muscle, fat and egg samples spiked at 0.25, 5 and 10 μ g g −1 levels were 82–130%. The FPIA results from analysis of incurred residues in chicken muscle samples showed that the simple procedure is viable.