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Quantitative determination of commercially relevant species in foods by real‐time PCR
Author(s) -
Laube Ines,
Zagon Jutta,
Broll Hermann
Publication year - 2007
Publication title -
international journal of food science and technology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.831
H-Index - 96
eISSN - 1365-2621
pISSN - 0950-5423
DOI - 10.1111/j.1365-2621.2006.01249.x
Subject(s) - taqman , food science , animal species , real time polymerase chain reaction , food products , biology , microbiology and biotechnology , zoology , biochemistry , gene
Summary Defined samples were examined regarding the proportion of beef, pork, lamb, goat, chicken and turkey utilising TaqMan TM PCR. A quantification was performed for determining the proportion of animal species in relation to the total proportion of meat in the food products. The adoption of published real‐time PCR systems (International Journal of Food Science and Technology, 2005) permitted a quantitative statement in processed meat products and in canned foods down to a concentration of 0.1%. Different factors influencing the amount of species determined were investigated.