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Contribution of Response Surface Design to the Synthesis of Monoacylglycerols Catalyzed by Rhizopus sp. lipase
Author(s) -
Koblitz Maria G.B.,
Pastore Glaucia M.
Publication year - 2005
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/j.1365-2621.2005.tb11508.x
Subject(s) - lipase , lauric acid , glycerol , yield (engineering) , molecular sieve , chemistry , molar ratio , substrate (aquarium) , catalysis , rhizopus arrhizus , central composite design , response surface methodology , chromatography , biocatalysis , sieve (category theory) , organic chemistry , nuclear chemistry , enzyme , materials science , biology , reaction mechanism , fatty acid , composite material , ecology , mathematics , combinatorics
The partially purified lipase from Rhizopus sp was tested as a biocatalyst for the synthesis of monolaurin from lauric acid and glycerol. A central composite rotatable design was applied to optimize the substrate molar ratio and the percentage of molecular sieve for achieving maximum monolaurin yield. The best results were obtained when the molar ratio of substrates was 1:1, the medium contained 100% (w/w) molecular sieve, and the reaction was conducted at 50 °C and 200 rpm applying 2 mg of the partially purified lipase. The maximum yield in monolaurin (17.52%) was achieved after a 72‐h reaction.