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Optimization of Thermal Pretreatment Conditions for the Separation of Native α‐Lactalbumin from Whey Protein Concentrates by Means of Selective Denaturation of β‐Lactoglobulin
Author(s) -
Tolkach Alexander,
Steinle Susanne,
Kulozik Ulrich
Publication year - 2005
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/j.1365-2621.2005.tb08319.x
Subject(s) - lactalbumin , chemistry , whey protein , lactose , denaturation (fissile materials) , alpha lactalbumin , chromatography , calcium , composition (language) , food science , nuclear chemistry , organic chemistry , linguistics , philosophy
In this study a method to obtain native α‐lactalbumin with a high degree of purity of 98% (m/m) and recovery of 75% (m/m) by selective denaturation of β‐lactoglobulin was developed. To achieve this goal, the thermal pretreatment of whey protein concentrate was optimized varying the composition of the liquid whey protein concentrate in terms of total protein, lactose and calcium content, and pH value. The kinetics of the thermal denaturation of α‐la and β‐lg were then investigated at predetermined optimal composition (protein content 5 to 20 g/L, lactose content 0.5 g/L, calcium content 0.55 g/L, and pH 7.5). Using the activation energies and reaction rate constants obtained, lines of equal effects for targeted denaturation degrees of α‐la and β‐lg were calculated. Depending on total protein content, an area of optimal heating temperature/time conditions was identified for each protein concentration level.