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Process Characteristics of Hydrolysis of Chitosan in a Continuous Enzymatic Membrane Reactor
Author(s) -
Kuo C.H.,
Chen C.C.,
Chiang B. H.
Publication year - 2004
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/j.1365-2621.2004.tb13638.x
Subject(s) - membrane reactor , chemistry , chromatography , batch reactor , membrane , immobilized enzyme , continuous stirred tank reactor , hydrolysis , chitobiose , continuous reactor , bacillus cereus , chitosan , enzyme , biochemistry , chitin , catalysis , biology , bacteria , genetics
Crude enzyme from Bacillus cereus NTU‐FC‐4 was used to hydrolyze chitosan of 66% deacetylation in a membrane reactor, operated at 45 °C and pH 5, to continuously produce chitooligosaccharides. Major oligomers in the product from the reactor were chitobiose, chitotriose, chitotetraose, chitopentaose, and chitohexaose. When the membrane reactor was operated at an enzyme/substrate ratio of 0.2 (unit/mg) and residence time of 100 min, it reached steady state in 2.5 h. The system could be operated for 15 h and still maintained a stable product composition. When the volume replacement exceeded 2.5, the productivity of the membrane reactor became higher than that of the batch reactor, and the difference between them became even greater when the volume replacement was further increased. The apparent Michaelis constant (K m ) for the enzyme in the membrane reactor was 18.8 mg/mL, but the apparent K m was 5.4 mg/mL for the batch reactor, suggesting that the affinity of the enzyme for chitosan was lower in the membrane reactor compared with the enzyme in the batch reactor. The estimated values of apparent V max were 0.18 and 0.20 mg reducing sugar/mL/min for the enzyme in the membrane reactor and in the batch reactor, respectively, indicating that the enzyme activity was not greatly altered when used in the membrane reactor.