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Optimization of Medium Composition for Cultivating Clostridium butyricum with Response Surface Methodology
Author(s) -
Kong Qing,
HE Guoqing,
Chen Qihe,
Chen Feng
Publication year - 2004
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/j.1365-2621.2004.tb13614.x
Subject(s) - clostridium butyricum , yeast extract , response surface methodology , food science , probiotic , ingredient , composition (language) , factorial experiment , agar , incubation , chemistry , bacteria , fractional factorial design , central composite design , agar plate , biology , fermentation , chromatography , biochemistry , mathematics , linguistics , philosophy , statistics , genetics
Probiotic bacteria, such as some strains of Clostridium butyricum , have been frequently used as the active ingredient in functional foods. However, slow growth of the probiotic bacteria has been one of the big concerns for their potential commercial application. In the present study, a fractional factorial design was applied to investigate the main factors, namely, the concentrations of the glucose, tryptone, yeast extract, beef extract, cys‐teine, (NH 4 ) 2 SO 4 , NaCl, K 2 HPO 4 , and the medium initial pH that affected the growth of 1 probiotic strain, C. butyricum ZJUCB, currently preserved in our laboratory. Central composite experimental design and response surface methodology were adopted to derive a statistical model for optimizing the composition of the medium. The experimental results showed that the optimum medium for incubating the C. butyricum ZJUCB was composed of 2.44% (w/v) glucose, 2.08% yeast extract (w/v), 1% tryptone (w/v), 0.1% (NH 4 ) 2 SO 4 (w/v), 0.1% NaHCO 3 (w/v), 0.02% MnSO 4 . H 2 O (w/v), 0.02% MgSO 4 . 7H 2 O (w/v), 0.002% CaCl 2 (w/v), and 2% agar (w/v) (if necessary) at pH 8.55. After incubation for 24 h in the optimum medium, the populations of the viable organisms could reach 10 9 colony‐forming units (CFU)/mL, which was 100 times higher than that incubated in the initial medium.