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An Improved Method for a Hydrophobic Grid‐membrane Filter System to Detect Heat‐injured Foodborne Pathogens in Ground Beef
Author(s) -
WU V. C. H.,
FUNG D. Y. C.
Publication year - 2004
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/j.1365-2621.2004.tb13376.x
Subject(s) - listeria monocytogenes , yersinia enterocolitica , salmonella , membrane filter , food science , escherichia coli , chemistry , microbiology and biotechnology , agar , membrane integrity , filter (signal processing) , contamination , membrane , chromatography , biology , bacteria , biochemistry , computer vision , ecology , genetics , gene , computer science
Recovery of heat‐injured pathogens ( Escherichia coli O157:H7, Listeria monocytogenes, Salmonella Typhimurium, and Yersinia enterocolitica ) in ground beef samples was studied by the thin agar layer (TAL) and TAL = Oxyrase® (TALO) methods in conjunction with the ISO‐GRID® hydrophobic grid‐membrane filter (HGMF). This new combination using ISO‐GRID HGMFs with the TAL and TALO methods improved the efficiency ( P < 0.05) of recovery of pathogens. In addition, the TALO method showed a higher percentage increase of recovery than the TAL method because of stimulation of Oxyrase in growth of pathogens. The combination allows the detection of very low numbers of pathogens in food.