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Identification of a Biomarker for the Detection of Prohibited Meat and Bone Meal Residues in Animal Feed
Author(s) -
Kim S.H.,
Huang T.S.,
Seymour T.A.,
Wei C.I.,
Kempf S.C.,
Bridgman C.R.,
Clemens R.A.,
An H.
Publication year - 2004
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/j.1365-2621.2004.tb09926.x
Subject(s) - meat and bone meal , immunostaining , biomarker , western blot , monoclonal antibody , chemistry , immunoassay , gel electrophoresis , microbiology and biotechnology , sodium dodecyl sulfate , antibody , chromatography , biology , biochemistry , immunohistochemistry , fish meal , gene , fishery , fish <actinopterygii> , immunology
A unique biomarker, h‐caldesmon, was identified and purified from bovine intestine smooth muscle. It was used to develop monoclonal antibodies (MAbs) for use in immunochemical assays to detect prohibited meat and bone meal (MBM) in animal feed. This biomarker with a molecular weight of 150 kDa was demonstrated by sodium dodecyl sulfate‐polyacrylamide gel electrophoresis (SDS‐PAGE) to be present in MBM samples that were obtained from different manufacturers. The presence of this biomarker in MBM and smooth muscle was also demonstrated by immunostaining with MAb 8B4 in Western blot assay. h‐Caldesmon in intestinal smooth muscle was demonstrated to be stable after autoclaving at 130 °C for up to 1 h. Because MAb 8B4 was sensitive in detecting MBM in animal feed at >0.05%, it can be used in immunoassays for MBM detection.