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Release of Nisin from Methylcellulose‐Hydroxypropyl Methylcellulose Film Formed on Low‐density Polyethylene Film
Author(s) -
Grower J. L.,
Cooksey K.,
Getty K.
Publication year - 2004
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/j.1365-2621.2004.tb06349.x
Subject(s) - nisin , listeria monocytogenes , chemistry , chromatography , food science , nuclear chemistry , bacteria , biology , organic chemistry , antimicrobial , genetics
Low‐density polyethylene film was coated with a solution containing a high‐molecular‐weight or low‐molecular‐weight methylcellulose and hydroxypropyl methylcellulose. Films contained 10000, 7500, 5000, 2500, or 0 IU/cm2 nisin. Film samples were placed into peptone water, and 10‐μL samples were removed and placed onto spiral plated lawns of Listeria monocytogenes . Zones of inhibition were measured using a caliper. Films containing 5000, 7500, and 10000 IU/cm2 nisin inhibited L. monocytogenes after 30 min; films with 7500 and 10000 IU/cm2 nisin inhibited L. monocytogenes after 60 min and 8 h, respectively. No zones of inhibition were observed after 24 h and 4 d for all films. After 8 d, zones of inhibition were observed for films with all levels of nisin except 2500 IU/cm2. Films with 0 and 2500 IU/ cm2 nisin did not produce zones of inhibition throughout the study. Molecular weight of the cellulose‐based carrier had no effect on inhibition of L. monocytogenes. A standard curve of inhibition was developed using solutions of 10000, 7500, 5000, 2500, or 0 IU/cm2 nisin applied directly to lawns of L. monocytogenes . The amount of inhibition using a direct application of a solution was 25% to 50% more effective for inhibition of L. monocytogenes compared with the coated film samples. Overall, the coated film samples were effective for inhibition of L. monocytogenes , particularly when 7500 and 10000 IU/cm2 nisin were used, but the release of nisin was not controlled and did not provide consistent inhibition throughout the 8‐d study.