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Fluorescent polarization immunoassay for sulphadiazine using a high specificity antibody
Author(s) -
Murtaziilya R.,
Eremin Sergei A.,
Mozoleva Olga V.,
Everest Sally J,
Jim Brown A,
Jackman Roy
Publication year - 2004
Publication title -
international journal of food science and technology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.831
H-Index - 96
eISSN - 1365-2621
pISSN - 0950-5423
DOI - 10.1111/j.1365-2621.2004.00862.x
Subject(s) - fluorescence polarization immunoassay , immunoassay , antiserum , chemistry , chromatography , fluorescein , fluorescence , detection limit , antibody , biology , immunology , physics , quantum mechanics
Summary A rapid and sensitive fluorescent‐labelled polarization immunoassay for sulphadiazine (SDZ) is reported. Fluorescein‐labelled tracers were synthesized and a specific SDZ antibody has been used in the development of the method. The influence of the fluorescent label type on the immunoassay sensitivity was investigated. Homologous and non‐homologous tracer – antiserum combinations were varied to provide increased sensitivity of the immunoassay. A detection limit of 0.2 ng mL −1 for SDZ in 50 μ L aqueous sample has been achieved. The cross‐reactivity of other sulphonamide drugs has also been examined.