Premium
The use of simultaneous 1 H & 31 P magic angle spinning nuclear magnetic resonance measurements to characterize energy metabolism during the conversion of muscle to meat
Author(s) -
Bertram Hanne C.,
Whittaker Andrew K.,
Andersen Henrik J.,
Karlsson Anders H.
Publication year - 2004
Publication title -
international journal of food science and technology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.831
H-Index - 96
eISSN - 1365-2621
pISSN - 0950-5423
DOI - 10.1111/j.1365-2621.2004.00826.x
Subject(s) - phosphocreatine , magic angle spinning , chemistry , nuclear magnetic resonance spectroscopy , glycogen , nuclear magnetic resonance , nmr spectra database , energy metabolism , analytical chemistry (journal) , spectral line , medicine , biochemistry , chromatography , organic chemistry , physics , astronomy
Summary In order to investigate the potential of magic angle spinning nuclear magnetic resonance (MAS NMR) in the elucidation of post‐mortem metabolism in muscle biopsies, simultaneous 1 H and 31 P MAS NMR measurements were made continuously on post‐mortem (20 min to 24 h) muscle longissimus samples from rabbits. The animals had either been or not been given adrenaline (0.5 mg kg −1 4 h pre‐slaughter) to deplete stores of muscle glycogen. The intracellular pH was calculated from 1 H spectra, and the post‐mortem rate of formation of lactate was followed and quantified. Comparison of measurements made on muscle samples from rabbits treated with adrenaline with measurements made on muscle samples from untreated rabbits revealed significant effects of adrenaline treatment on both pH (pH24 h = 6.42 vs. pH24 h = 5.60) and formation of lactate (16 mmol g −1 vs. 65 mmol g −1 ). The 31 P NMR spectra were used to follow the rate of degradation of ATP and phosphocreatine. The present study clearly shows that MAS NMR has potential for the study of post‐mortem energy metabolism.