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Development of a Monoclonal Antibody for Grouper ( Epinephelus marginatus ) and Wreck Fish ( Polyprion americanus ) Authentication Using an Indirect ELISA
Author(s) -
Asensio L.,
González I.,
Rodríguez M.A.,
Hernández P.E.,
García T.,
Martín R.
Publication year - 2003
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/j.1365-2621.2003.tb06990.x
Subject(s) - grouper , epinephelus , biology , monoclonal antibody , fish <actinopterygii> , fishery , zoology , antibody , immunology
A monoclonal antibody generated against soluble muscle proteins from grouper ( Epinephelus marginatus ) has been used in 2 indirect ELISA formats (microtiter plates and immunostick tubes) for the rapid authentication of grouper and wreck fish ( Polyprion americanus ). This monoclonal antibody (1A4‐MAb) was tested against native and heat‐treated (cooked and sterilized) soluble muscle protein extracts from several commonly marketed fish species and only reacted with grouper and wreck fish samples.