Premium
Antioxidative Effects of Glycoprotein Isolated from Solanum nigrum Linne on Oxygen Radicals and its Cytotoxic Effects on the MCF‐7 Cell
Author(s) -
Lee S.J.,
Lim K.T.
Publication year - 2003
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/j.1365-2621.2003.tb05695.x
Subject(s) - glycoprotein , chemistry , radical , cytotoxic t cell , superoxide , cytotoxicity , in vitro , reactive oxygen species , p glycoprotein , biochemistry , mcf 7 , hydroxyl radical , microbiology and biotechnology , biology , enzyme , cancer cell , multiple drug resistance , antibiotics , human breast , cancer , genetics
Glycoprotein of Solanum nigrum Linne (SNL glycoprotein) was isolated and identified using SDS‐PAGE. SNL glycoprotein's antioxidative effects were tested in vitro and its cytotoxicity effects were tested using MCF‐7 cells. One glycoprotein was isolated from SNL fruits, the other from stems and leaves. SNL glycoprotein is reactive with oxygen radicals, such as the ‐OH and O 2 ‐ in vitro. Moreover, the SNL glycoprotein's radical scavenging activity is sensitive to the superoxide anion radical and the hydroxyl radical. In the MCF‐7 cell, SNL glycoprotein I had a cytotoxic effect at 1 μg/mL and SNL glycoprotein II at 100 μg/mL. When MCF‐7 cells were treated with SNL glycoproteins, nitrite oxide production was induced.