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Development of a Simple Method for Detecting Presumptive Escherichia coli on Fresh Retail Beef
Author(s) -
Bolling B.W.,
Schmidt D.J.,
Ingham S.C.
Publication year - 2002
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/j.1365-2621.2002.tb11394.x
Subject(s) - escherichia coli , diluent , food science , chemistry , chromatography , mathematics , nuclear chemistry , biochemistry , gene
A simple method of detecting Escherichia coli on retail beef was developed. Diluent used in sponge‐sampling of meat was added to a tube containing double‐strength lauryl sulfate tryptone broth + 5‐bromo‐4‐chloro‐3‐indolyl β‐D‐glucuronide (X‐GlcA) and a Durham tube. Tubes with blue color and gas after 24 h incubation at 35 °C were presumptive‐positive. Chi‐squared analysis showed a significant ( P < 0.05) relationship between the method and the Petrifilm™ E. coli /Coliform Count Plate method for E. coli in analysis of 66 retail beef samples and the 2 methods had a similar proportion of confirmed isolates (61 and 63%). The method developed is qualitatively comparable to the Petrifilm™ method and provides small‐scale beef processors a simple way to monitor sanitary conditions in fabrication and grinding operations.