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Response Surface Modeling of Lipase‐Catalyzed Isoamyl Propionate Synthesis
Author(s) -
Hari Krishna S.,
Karanth N.G.
Publication year - 2002
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/j.1365-2621.2002.tb11354.x
Subject(s) - substrate (aquarium) , propionate , yield (engineering) , response surface methodology , lipase , chemistry , central composite design , isoamyl acetate , catalysis , matrix (chemical analysis) , chemical engineering , chromatography , enzyme , organic chemistry , materials science , composite material , oceanography , engineering , ethyl acetate , geology
Response surface methodology based on a 3‐variable, 5‐level central composite rotatable design matrix was used to optimize the synthesis. The influence of selected variables, namely, enzyme/substrate (E/S) ratio, substrate concentration, and temperature was investigated. E/S ratio and temperature had a strong effect on esterification, while substrate concentration did not. Reaction time did not show significant effect on ester yields, and conversions could be completed in less than 24 h. The response surface model has been found to describe the experimental results adequately. Product yield at optimum conditions (24 g/mol E/S ratio, 1.5 M substrate concentration 20 °C) was 1.4 M, representing 93% conversion, which is in good agreement with the predicted one.