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Edible Antimicrobial Films Based on Chitosan Matrix
Author(s) -
Coma V.,
MartialGros A.,
Garreau S.,
Copinet A.,
Salin F.,
Deschamps A.
Publication year - 2002
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/j.1365-2621.2002.tb09470.x
Subject(s) - chitosan , listeria monocytogenes , food science , antimicrobial , flocculation , chemistry , listeria , strain (injury) , matrix (chemical analysis) , microbiology and biotechnology , bacteria , chromatography , biochemistry , biology , organic chemistry , genetics , anatomy
: Edible chitosan coatings showed anti‐ Listeria monocytogenes effect evaluated by numeration and epifluorescence methods, imparting a strong localized functional effect at the food surface by active packaging. The use of film‐forming solution in culture liquid medium showed a known flocculant phenomenon combined with bactericidal activity, keeping 20% of the initial microbial charge as viable cells in flocculant, which could develop subsequently. However, chitosan film showed 100% of L. monocytogenes inhibition for at least 8 d, completed by bactericidal activity measured by epifluorescence assays. A decrease in antibactericidal effect with time was obtained, most probably due to a decreasing availability of amino‐groups of chitosan. Latter results were validated on Emmental cheese samples using L. innocua as model strain because of its nonpathogenicity.