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Electrophoretic Identification of Muscle Proteins in 7 Puffer Species
Author(s) -
Chen T.Y.,
Hwang D.F.
Publication year - 2002
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/j.1365-2621.2002.tb09431.x
Subject(s) - coomassie brilliant blue , staining , silver stain , polyacrylamide gel electrophoresis , sodium dodecyl sulfate , electrophoresis , chemistry , gel electrophoresis , myofibril , urea , gel electrophoresis of proteins , chromatography , biochemistry , species identification , biology , microbiology and biotechnology , enzyme , genetics
Species identification of puffer species using sodium dodecyl sulfate‐polyacrylamide gel electrophoresis (SDS‐PAGE) with Coomassie blue and Coomassie blue/silver double staining was performed. A comparison was also made among extracts of sarcoplasmic, myofibrillar, SDS‐soluble, and urea‐soluble proteins. Protein bands of lower molecular weight showed the species‐specific characteristics. Double staining better discriminated the protein banding patterns of different puffer species than Coomassie blue staining. Species identification of all tested puffers could be achieved by judging from the SDS‐PAGE patterns of the 4 protein extracts stained with both stains along with protein band densities.