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Simultaneous Analysis of Tocopherols, Cholesterol, and Phytosterols Using Gas Chromatography
Author(s) -
Du M.,
Ahn D.U.
Publication year - 2002
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/j.1365-2621.2002.tb08708.x
Subject(s) - saponification , stigmasterol , chemistry , chromatography , gas chromatography , trimethylsilyl , hexane , tocopherol , phytosterol , cholesterol , campesterol , extraction (chemistry) , sterol , organic chemistry , vitamin e , biochemistry , antioxidant
A method for the simultaneous analysis of tocopherols and sterols was developed. Lipids were extracted with the Folch method, saponified by KOH‐ethanol, and then tocopherols, cholesterol, and phytosterols were extracted with hexane. The extracted samples were dried under a nitrogen stream, derivatized using trimethylsilyl compounds, and then subjected to a gas chromatography. The recovery rates for cholesterol, stigmasterol, and sitosterol were about 100%, but recovery rates for tocopherols were low (25% forδtocopherol and 66% for γ‐tocopherol) and varied according to compound structures. However, the recovery rates for δ‐ and γ‐tocopherols increased to about 100% when the amounts of water and hexane were increased to 15 mL at the extraction step after saponification.