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An Automated Flow Injection Analysis Procedure for the Determination of Reducing Sugars by DNSA Method
Author(s) -
CańtizaresMacías P.,
HernándezGarciadiego L.,
GómezRuíz H.
Publication year - 2001
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/j.1365-2621.2001.tb16118.x
Subject(s) - reagent , chemistry , calibration , standard addition , chromatography , analytical chemistry (journal) , standard solution , tartrate , flow injection analysis , inorganic chemistry , organic chemistry , detection limit , mathematics , statistics
A nonenzymatic spectrophotometric method, coupled to an automatic system of standard additions, based on the reaction with 3,5‐dinitrosalicylic acid (DNSA) is described. In a system based on an exhaustive reaction, sample volumes are introduced together with calibration solutions, thus the calibration is performed in a nonsegmented flow system. Homogenization of the sample/standard/carrier takes place in the calibration loop. Later, it is injected in the flow injection analysis system, where the reaction with DNSA occurs, to obtain 3,5‐diaminosalicylic acid which is measured spectrophotometrically. With the proposed method, it is possible to eliminate the Rochelle salt, sodium and potassium tartrate, phenol and sodium bisulfite of the principal reagent. Sample throughput was 11 samples/h and precision, expressed as relative standard deviation, was 2.3%.